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BIOL 335LMolecular Biology Techniques

Units
0.50
Faculty
Science
Subject
Biology

When it runs

  1. F24
  2. W25
  3. S25
  4. F25
  5. W26
  6. S26
  7. F26
  8. W27
  9. S27

Ran in 5 of the last 7 terms. Winter 2027: scheduled. Spring 2027: schedule not out yet.

Selected experiments to provide students with a range of laboratory skills in recombinant DNA technology.

Prerequisites

  • Complete all of the following
    • Must have completed the following:
      • BIOL240L - Microbiology Laboratory (0.25)
    • Complete 1 of the following
      • Must have completed the following:
        • BIOL235 - Foundations of Molecular Biology (0.50)
      • Must have completed the following:

Antirequisites

None listed.

Leads to

No course lists it as a requisite.

Fall 2026 sections

Enrolment from UW Open Data, which can trail Quest by up to two days. Instructors and rooms: Schedule of Classes.
SectionClassEnrolledMeets
LAB 001696417 / 22W 14:30–19:20
LAB 002696520 / 22Th 14:30–19:20
TUT 101696637 / 44M 13:30–14:20

Winter 2027 sections

Enrolment from UW Open Data, which can trail Quest by up to two days. Instructors and rooms: Schedule of Classes.
SectionClassEnrolledMeets
LAB 00167600 / 22W 14:30–18:20
LAB 00267610 / 22Th 14:30–18:20
LAB 701248327 / 9999Online or by arrangement
TUT 10167620 / 44M 12:30–14:20

Reviewed on UW Flow

100% liked it94% easy100% useful

From 13 ratings and 5 comments on UW Flow. The most helpful comments, quoted as written, without names. Read them all on UW Flow.

  • Very easy lab course. Lots of downtime and never really in a rush. Easy to leave early if you're well-prepared and/or have previous experience doing this for a co-op. Worst part would be having to work on a research project development/proposal throughout the term.

    Apr 2019 · liked it · 1 found this helpfulOn UW FlowRemove this

  • Very fun course, depends a lot on the TAs you get as you spend a lot of time under their instruction. Laura is very knowledgeable and kind. Exams are very easy with a bit of studying and the final project feels like real research compared to other lab reports. Highly recommend to anyone going into bio research.

    Sep 2025 · liked itOn UW FlowRemove this

  • REALLY useful wet labs. The major techniques covered are: making and performing gel electrophoresis from scratch, Safeview gel imaging, DNA purification and analysis, PCR, DNA RE digestion + ligation to a vector, cell transformation (heat shock and electroporation), protein SDS electrophoresis, protein western blotting/probing. These techniques will be extremely useful for coops and research positions. The course grading is: lab performance (20%), lab quizzes (3%), lab notebook checks (20%), experimental design proposal (12%), 2 midterms (20% and 25%). The experimental design proposal is done with your lab partner. You are given a research scenario/prompt and will follow weekly milestone…

    Dec 2023 · liked itOn UW FlowRemove this

  • 6 hour lab. Very useful! Learn to set up/run agarose and SDS PAGE gels, western blots, make your own petri dishes, transform bacteria with recombinant plasmids, etc.

    Jul 2018 · liked itOn UW FlowRemove this

  • Loved the lab, loved the course. Very very useful in the real world.

    Apr 2015 · liked itOn UW FlowRemove this

Discussed on r/uwaterloo

22 posts and comments in 19 threads mention BIOL 335L, April 2013 to December 2024. Quoted word for word, newest first; each reply sits under the post and the comment that started its branch. Search r/uwaterloo for newer posts.

Fall 2023

Spring 2023

Winter 2022

Spring 2021

Am I crazy? 0 points, 17 comments

u/buttercupbeuaty: “… willing to drop biol 309 bc I dropped it before after doing terribly I just wanted to take biol 335l in the winter. And I can’t drop my lab and computer based courses since they’re so useful I’ve done 7-8 courses at once before so am I crazy? …”

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Fall 2020

Exciting courses 6 points, 20 comments

“Is anyone else enrolled in any courses you find exciting next semester? I'm super excited about a few of mine next term.”

u/VenomRex: “what are the exciting courses you are talking about?”

[deleted]: “For me, they're exciting. It's virology (biol 442), molecular biology techniques (biol 335l) and advanced immunology (biol 441).”

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Spring 2020

BIOL 335L VS CHEM 335L 1 point, 6 comments

u/RonaSeason123: “I'm looking to do one or the other but don't know which lab is more useful for jobs in the pharmaceutical industry. Any insight would be helpful! FYI, I am a medicinal chemistry student.”

u/b266curve: “Not sure if you are looking into taking one of them this fall, but BIOL 335L is not offered for the Fall 2020 term (it was cancelled). It is offered in the winter though! I can't find any information about CHEM 335L, however.”

u/boyidgaf: “Take both if you can, but Chem 335L might be a bit more relevant.”

u/RonaSeason123: “I have to fit Chem 357 into my schedule to take Chem 335L. If I can only take Biol 335L would that still be good tho? If not then I can squeeze Chem 335L.”

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Winter 2020

Fall 2019

Fall 2018

Need help picking elective for 2B 4 points, 3 comments

“Anyone pls, toss me some suggestions. I'm in science so something science related would be nice or somethings that's interesting and easy. Thanks :3”

u/hoedat: “biol 241-interesting, useful if you like that stuff biol 335l-useful, was fun lab, tutorials were boring econ 101-fun + easy clas 104-fun + easy chem 224l-useful chem 221-useful + easy”

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Winter 2018

Come crush my dreams pls 7 points, 35 comments

“I'm an honors science student and just finished my first year of uni... my marks were okay, about mid to low 80's mostly but I know that most professional programs require higher marks than what I …”

u/Reikki: “… I recommend taking BIOL 439, BIOL 335L for mark boosters! …”

u/Theblastmaster: “If you want to do research and learn real techniques molecular biologists use 335L is a must. The most useful course I've taken maybe except BIOL 309, because BIOL 335L is essentially just BIOL 309L.”

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Fall 2017

BIOL 335L and BIOL 348L 1 point, 2 comments

u/TheBestOfTheGreats: “Hey guys and gals, just wondering whether anyone here has taken either 335L or 348L? I want to take one of them, but they both sound interesting. Anyone that's taken either of the courses do you have any positive or negative experiences?”

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Check it elsewhere for Fall 2026

Calendar versions

Undergraduate Calendar